| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Chlorobium limicola | GAAATTGCAGCCGTTCCCC | AACCGGAACCGCTCGAAA | 59.78 | 59.58 | 268 | 137 |
100.00%
|
100.00%
|
| Primer 12 | Chlorobium limicola | AGCTGCGCTTTCATCCCA | ACAGCTGAGCCGTTCTGTC | 59.65 | 60.01 | 179 | 135 |
100.00%
|
100.00%
|
| Primer 13 | Chlorobium limicola | AGCTGCGCTTTCATCCCA | ACAGCTGAGCCGTTCTGT | 59.65 | 58.86 | 179 | 135 |
100.00%
|
100.00%
|
| Primer 14 | Chlorobium limicola | CAGGAAACACCGTCAGCCA | AAAACCAAGCACCGCACC | 60.23 | 59.19 | 201 | 135 |
100.00%
|
100.00%
|
| Primer 15 | Chlorobium limicola | AGGAAACACCGTCAGCCA | AAAACCAAGCACCGCACC | 58.76 | 59.19 | 200 | 135 |
100.00%
|
100.00%
|
| Primer 16 | Chlorobium limicola | TGAGTGCGCATTCTGCCA | CGGAGTGCTGCAGCTTTTG | 59.97 | 60.08 | 298 | 135 |
100.00%
|
100.00%
|
| Primer 17 | Chlorobium limicola | TTCCCGACGTAAAGGCTGC | TCGGTTCTGATGCGGATGC | 60.38 | 60.52 | 277 | 135 |
100.00%
|
100.00%
|
| Primer 18 | Chlorobium limicola | ACCTTCCGGTTCGCGATAC | TGATGAGCGCAACCTGCA | 59.86 | 59.97 | 219 | 135 |
100.00%
|
100.00%
|
| Primer 19 | Chlorobium limicola | AGCTGCGCTTTCATCCCA | CAGCTGAGCCGTTCTGTCA | 59.65 | 60.01 | 178 | 132 |
100.00%
|
100.00%
|
| Primer 20 | Chlorobium limicola | TATGTGGTTGCGACGCCA | TGAGCGTGCGAACCATGA | 59.66 | 59.66 | 289 | 132 |
100.00%
|
100.00%
|